HER 141


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Name
MP13
Morphotype
A1 (Myophage)
Realm
Duplodnaviria
Kingdom
Heunggongvirae
Phylum
Uroviricota
Class
Caudoviricetes
Electron Micrograph
Image
Image of phage under microscope
Image description
Magnification: 297,000X
Bar: 50 nm
Staining: UAB
Characteristics

Plaques: 0.5mm, clear-turbid, halo, ring.
Inactivated after 8 years in broth at -70oC.
Sensitive to chloroform.
Generalized transducing phage (lytic) for QM B1551.
Estimate of molecular weight (restriction fragments: 97x10^6 dalton. 20% of thymine replaced by some form of substitued HMU.
Dimensions in nm (Ackermann unpubl.):
UA head 93 tail 199 x 21
PT head 93 tail 199 x 15

Genomic sequence
No
Bacterial hosts
Reference
Vary, P. S. 1979. BBRC 88:1119-1124.
Remarks
To be deposited (1982) in:
*Bacillus* Genetic Stock Center,
Ohio State University,
Columbus, OH, 43210
Vary: mid-log cells, MOI = 1, shake at 22 rpm for 2-3 h.
Instructions for phage propagation are available upon request.
History
Received from
Date
Dr Patricia S. Vary,
Department of biological Sciences,
Northern Illinois University,
DeKalb, Illinois, 60115,
USA.
09-01-1982
Isolated by
Date
Dr Patricia S. Vary
02-1977
Source
Soil, Chicago, Illinois, USA 2-1977.
Updated at
2024-01-16