HER 313


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Name
η (eta)
Morphotype
B1 (Siphophage)
Family
?1
Electron Micrograph
Image
Image of phage under microscope
Image description
Magnification: 297,000X
Bar: 50 nm
Staining: UA
Characteristics
Clear plaques of 0.5mm
Buoyant density in CsCl = 1.495g/mL
Bacterial hosts
Reference
Von Lohr, R. 1970. Z. Allg. Mikrobiol. 10:269-274. (En allemand/ In deutsch)
Pons, F.W. 1966. Biochem. Z. 346:26-40. (En allemand/ In deutsch)
Remarks
Originally described as short tailed.
Guanine partially replaced by unknown base; DNA contains nonidentified sugar.
Infects log-phase cells only.
Adsorbs well, forms large, moderately turbid plaques from which lysogens can be isolated. Pre-adsorption is not required for plating.
Phage inoculum for double-layer agar 15 000 PFU, yield 10^11 PFU.
Multiplies on *S. marcescens* CV (σ)
History
Received from
Date
R.W. Kaplan
Received from
Date
Dr F.W. Pons
Institut fér Mikrobiologie
Johann Wolfgang Goethe-Universitat
Theodor-Stern-Kai 7, Haus 75A
D-6000 Frankfurt am Main, Germany
03-03-1988
Isolated by
Date
R.W. Kaplan
Institut fér Mikrobiologie
Frankfurt, Germany
1960
Source
Supernatant of an overnight culture of a lost strain : *S. marcescens* ES
Updated at
2024-01-19