HER 141
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Name
MP13
Morphotype
A1 (Myophage)
Family
?1
Electron Micrograph
Image
Image description
Magnification: 297,000X
Bar: 50 nm
Staining: UAB
Bar: 50 nm
Staining: UAB
Characteristics
Plaques: 0.5mm, clear-turbid, halo, ring.
Inactivated after 8 years in broth at -70oC.
Sensitive to chloroform.
Generalized transducing phage (lytic) for QM B1551.
Estimate of molecular weight (restriction fragments: 97x10^6 dalton. 20% of thymine replaced by some form of substitued HMU.
Dimensions in nm (Ackermann unpubl.):
UA head 93 tail 199 x 21
PT head 93 tail 199 x 15
Inactivated after 8 years in broth at -70oC.
Sensitive to chloroform.
Generalized transducing phage (lytic) for QM B1551.
Estimate of molecular weight (restriction fragments: 97x10^6 dalton. 20% of thymine replaced by some form of substitued HMU.
Dimensions in nm (Ackermann unpubl.):
UA head 93 tail 199 x 21
PT head 93 tail 199 x 15
Genomic sequence
No
Bacterial hosts
HER
Reference
Vary, P. S. 1979. BBRC 88:1119-1124.
Remarks
To be deposited (1982) in:
*Bacillus* Genetic Stock Center,
Ohio State University,
Columbus, OH, 43210
Vary: mid-log cells, MOI = 1, shake at 22 rpm for 2-3 h.
Instructions for phage propagation are available upon request.
*Bacillus* Genetic Stock Center,
Ohio State University,
Columbus, OH, 43210
Vary: mid-log cells, MOI = 1, shake at 22 rpm for 2-3 h.
Instructions for phage propagation are available upon request.
History
Isolated by
Date
Dr Patricia S. Vary
Received from
Date
Dr Patricia S. Vary,
Department of biological Sciences,
Northern Illinois University,
DeKalb, Illinois, 60115,
USA.
Department of biological Sciences,
Northern Illinois University,
DeKalb, Illinois, 60115,
USA.
1-9-1982
Source
Soil, Chicago, Illinois, USA 2-1977.
Updated at
2024-01-16