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HER Number
141
Identification
Name
Morphotype
MP13
A1 (Myophage)
Order
Family
Genus
Species
Other designations
Electron Micrograph
Magnification: 297,000X
Bar: 50 nm
Staining: UAB
Restriction profile
Characteristics
Complete genome sequence
Plaques: 0.5mm, clear-turbid, halo, ring.
Inactivated after 8 years in broth at -70oC.
Sensitive to chloroform.
Generalized transducing phage (lytic) for QM B1551.
Estimate of molecular weight (restriction fragments: 97x10^6 dalton. 20% of thymine replaced by some form of substitued HMU.
Dimensions in nm (Ackermann unpubl.):
UA head 93 tail 199 x 21
PT head 93 tail 199 x 15
Non
Propagation conditions
Host strain HER
1141
Temp.
Medium
Aerobic/Anaerobic
Agitation
Others
25-30ºC
BHI, TSA
200 rpm
Original reference
Vary, P. S. 1979. BBRC 88:1119-1124.
Remarks
To be deposited (1982) in:
Bacillus
Genetic Stock Center,
Ohio State University,
Columbus, OH, 43210
Vary: mid-log cells, MOI = 1, shake at 22 rpm for 2-3 h.
Instructions for phage propagation are available upon request.
History
Isolé par
Date
Dr Patricia S. Vary
Received from
Date
Dr Patricia S. Vary,
Department of biological Sciences,
Northern Illinois University,
DeKalb, Illinois, 60115,
USA.
1-9-1982
Original source
Soil, Chicago, Illinois, USA 2-1977.
Last update
2024-01-16
GREB, Pavillon de Médecine Dentaire
Université Laval, QC, Canada, G1V 0A6
Téléphone :
418-656-2485 Fax : 418-656-2861
Print
Ce site utilise
TYPO3
Réalisation:
Équipe Tactic