HER Number
141 Add to cart

Identification
Name Morphotype
MP13 A1
Order Family Genus Species
Caudovirales Myoviridae MP13
Other designations

Electron Micrograph
Magnification: 297,000X
Bar: 50 nm
Staining: UAB
Restriction profile

Characteristics Complete genome sequence
Plaques: 0.5mm, clear-turbid, halo, ring.
Inactivated after 8 years in broth at -70oC.
Sensitive to chloroform.
Generalized transducing phage (lytic) for QM B1551.
Estimate of molecular weight (restriction fragments: 97x10^6 dalton. 20% of thymine replaced by some form of substitued HMU.
Dimensions in nm (Ackermann unpubl.):
UA head 93 tail 199 x 21
PT head 93 tail 199 x 15
Non

Propagation conditions
Host strain HER
1141
Temp. Medium Aerobic/Anaerobic Agitation Others
25-30ºC BHI, TSA 200 rpm

Original reference
Vary, P. S. 1979. BBRC 88:1119-1124.

Remarks
To be deposited (1982) in:
Bacillus Genetic Stock Center,
Ohio State University,
Columbus, OH, 43210
Vary: mid-log cells, MOI = 1, shake at 22 rpm for 2-3 h.
Instructions for phage propagation are available upon request.

History
Isolated by Date
Dr Patricia S. Vary
Received from Date
Dr Patricia S. Vary,
Department of biological Sciences,
Northern Illinois University,
DeKalb, Illinois, 60115,
USA.
1-9-1982
Original source
Soil, Chicago, Illinois, USA 2-1977.

Last update
2006-05-15
GREB, Pavillon de Médecine Dentaire
Université Laval, QC, Canada, G1V 0A6
Téléphone : 418-656-2485 Fax : 418-656-2861
Ce site utilise TYPO3 Réalisation: Équipe Tactic