HER Number
158 Add to cart

Identification
Name Morphotype
RB69 A2
Order Family Genus Species
Caudovirales Myoviridae T4-like viruses
Other designations

Electron Micrograph
Magnification: 297,000X
Bar: 50 nm
Staining: PT
Restriction profile

Characteristics Complete genome sequence
Plaques: 1mm, clear.
Propagation on HER 1037 ?
Relationships with T4 in genes 31, ndd, 43.
Dimensions:
PT: head 106 x 77 nm Tail 109 x 17 nm
UA: head 112 x 80 nm Tail 110 x 20 nm
Oui
https://www.ncbi.nlm.nih.gov/nuccore/32453484

Propagation conditions
Host strain HER
1040
Temp. Medium Aerobic/Anaerobic Agitation Others
37ºC BHI, TSA

Original reference
Yeh L. S., T. Hsu, J. D. Karam. 1998. Divergence of a DNA Replication Gene Cluster in the T4-Related Bacteriophage RB69. J Bacteriol. 180: 2005-2013.

Remarks
Also held in the collection of J. Karam, Eric Miller and Larry Gold.
PT: head 106 x 77 nm Tail 109 x 17 nm
UA: head 112 x 80 nm Tail 110 x 20 nm
Diameter of capsid, 2 nm; collar, 24 x 2 nm; neck, 8 x 7 nm; tail tube, 105 x 7 nm,
noncontracted sheath, 99 x 17 nm; contracted sheath, 35 x 23 nm; base plate, 32 x 3 nm; spikes, 12 x 3nm; fibers, 133 x 2 nm with kink at 68 nm.
Extended tails had 24 striations; the number of tail fibers was 6.

History
Isolated by Date
Rosina O. Berry,
undergraduate student with R. L. Russell,
Cold Spring Harbor Laboratory
Received from Date
Dr. Jan Drake,
National Institute of Environmental Health Sciences (NIEHS),
Research Triangle Park, NC, 27709,
USA.
Received from Date
Dr William B. Wood,
University of Colorado,
Boulder, CO.
Who received it from:
R. L. Russell and R. S. Edgar
Received from Date
Dr Jim Karam,
Tulane University School of Medecine,
1430 Tulane Avenue,
New Orleans, LA, 70112,
USA.
Original source

Last update
2017-08-30
GREB, Pavillon de Médecine Dentaire
Université Laval, QC, Canada, G1V 0A6
Téléphone : 418-656-2485 Fax : 418-656-2861
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