HER Number
312 Add to cart

Identification
Name Morphotype
σ (sigma) B1
Order Family Genus Species
Caudovirales Siphoviridae
Other designations

Electron Micrograph
Magnification: 297,000X
Bar: 50 nm
Staining: UA
Restriction profile

Characteristics Complete genome sequence
Clear plaques of 0.5mm
Buoyant density in CsCl = 1.515g/mL
Non

Propagation conditions
Host strain HER
1311
Temp. Medium Aerobic/Anaerobic Agitation Others
30ºC TSA

Original reference
Von Lohr, R. 1970. Z. Allg. Mikrobiol. 10:269-274. (En allemand/In deutsch)

Remarks
Adsorbs poorly, forms small, irregular, turbid plaques from which lysogens can be isolated. Prophage is not UV-inducible.
Pre-adsorption to the indicator bacterium is recommended for plating.
Spontaneous clear-plaque mutants are very frequent (10^-4) and should be used for preparing high titer lysates.
The phage is able to multiply on S. marcescens CV(η).

History
Received from Date
Dr R.W. Kaplan
Institut für Mikrobiologie
Frankfurt, Germany
Received from Date
Dr F.W. Pons
Institut für Mikrobiologie
Johann Wolfgang Goethe-Universitat
Theodor-Stern-Kai 7, Haus 75A
D-6000 Frankfurt am Main, Germany
03-03-1988
Isolated by Date
Dr R.W. Kaplan 1960
Original source
Supernatant of an overnight culture of a lost strain : S. marcescens ES

Last update
2006-04-13
GREB, Pavillon de Médecine Dentaire
Université Laval, QC, Canada, G1V 0A6
Téléphone : 418-656-2485 Fax : 418-656-2861
Ce site utilise TYPO3 Réalisation: Équipe Tactic