4-Salt nutrient broth

Détails

For 1 liter of 4-salt:
10 g of Nutrient broth.
5 g of Peptone.
2.5 g of Yeast extract.
9.35 g of NaCl (0.16M).
0.283 g of KCl (3.8mM).
4.44 g of MgSO4.7H2O (0.018M).
0.559 g of CaCl2.2H2O (3.8mM).
Complete to 1 liter with water.

For 4-salt agar:
Add 10 g of agar per liter.