LB

For 1 liter of LB:
10 g of Bacto tryptone.
5 g of yeast extract.
5 g of NaCl.
Complete to 1 liter with water.

For LB-agar:
Add 15 g of agar per liter.

For LB-top agar:
Add 7.5 g of agar per liter.

Autoclave.

Add 10 mM of sterile CaCl₂ and 10 mM of sterile MgCl₂ after autoclave

Last modifications: 2011-01-17