HER 312
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Name
Σ (sigma)
Morphotype
B1 (Siphophage)
Family
?1
Electron Micrograph
Image
Image description
Magnification: 297,000X
Bar: 50 nm
Staining: UA
Bar: 50 nm
Staining: UA
Characteristics
Clear plaques of 0.5mm
Buoyant density in CsCl = 1.515g/mL
Buoyant density in CsCl = 1.515g/mL
Genomic sequence
No
Bacterial hosts
HER
Reference
Von Lohr, R. 1970. Z. Allg. Mikrobiol. 10:269-274. (En allemand/In deutsch)
Remarks
Adsorbs poorly, forms small, irregular, turbid plaques from which lysogens can be isolated. Prophage is not UV-inducible.
Pre-adsorption to the indicator bacterium is recommended for plating.
Spontaneous clear-plaque mutants are very frequent (10^-4) and should be used for preparing high titer lysates.
The phage is able to multiply on *S. marcescens* CV(?).
Pre-adsorption to the indicator bacterium is recommended for plating.
Spontaneous clear-plaque mutants are very frequent (10^-4) and should be used for preparing high titer lysates.
The phage is able to multiply on *S. marcescens* CV(?).
History
Received from
Date
Dr R.W. Kaplan
Institut für Mikrobiologie
Frankfurt, Germany
Institut für Mikrobiologie
Frankfurt, Germany
Received from
Date
Dr F.W. Pons
Institut für Mikrobiologie
Johann Wolfgang Goethe-Universitat
Theodor-Stern-Kai 7, Haus 75A
D-6000 Frankfurt am Main, Germany
Institut für Mikrobiologie
Johann Wolfgang Goethe-Universitat
Theodor-Stern-Kai 7, Haus 75A
D-6000 Frankfurt am Main, Germany
03-03-1988
Isolated by
Date
Dr R.W. Kaplan
1960
Source
Supernatant of an overnight culture of a lost strain : *S. marcescens* ES
Updated at
2024-01-19