HER Number
482 Add to cart

Identification
Name Morphotype
A2 Siphophage
Order Family Genus Species
Ashduovirus Ashduovirus A2
Other designations
Φ393-A2

Electron Micrograph
Restriction profile

Characteristics Complete genome sequence
Oui
https://www.ncbi.nlm.nih.gov/nuccore/NC_004112.1

Propagation conditions
Host strain HER
1482
Temp. Medium Aerobic/Anaerobic Agitation Others
37ºC MRS 10mM CaCl2, 10mM MgCl2

Original reference
1. Herrero, M., C. G. Delosreyesgavilan, J. L. Caso, and J. E. Suarez. 1994. Characterization of Φ393-A2, a bacteriophage that infects Lactobacillus casei. Microbiology-(UK) 140:2585-2590.
2. Garcia, P., J. C. Alonso, and J. E. Suarez. 1997. Molecular analysis of the cos region of the Lactobacillus casei bacteriophage A2. Gene product 3, gp3, specifically binds to its downstream cos region. Mol. Microbiol. 23:505-514.
3. Garcia, P., V. Bascaran, A. Rodriguez, and J. E. Suarez. 1997. Isolation and characterization of promoters from the Lactobacillus casei temperate bacteriophage A2. Can. J. Microbiol. 43:1063-1068.
4. Alvarez, M. A., M. Herrero, and J. E. Suarez. 1998. The site-specific recombination system of the Lactobacillus species bacteriophage A2 integrates in Gram-positive and Gram-negative bacteria. Virology 250:185-193.
5. Ladero, V., P. Garcia, V. Bascaran, M. Herrero, M. A. Alvarez, and J. E. Suarez. 1998. Identification of the repressor-encoding gene of the Lactobacillus bacteriophage A2. J. Bacteriol. 180:3474-6.
6. Alvarez, M. A., A. Rodriguez, and J. E. Suarez. 1999. Stable expression of the Lactobacillus casei bacteriophage A2 repressor blocks phage propagation during milk fermentation. J. Appl. Microbiol. 86:812-816.
7. Garcia, P., V. Ladero, J. C. Alonso, and J. E. Suarez. 1999. Cooperative interaction of CI protein regulates lysogeny of Lactobacillus casei by bacteriophage A2. J. Virol. 73:3920-3929.
8. Ladero, V., P. Garcia, J. C. Alonso, and J. E. Suarez. 1999. A2 Cro, the lysogenic cycle repressor, specifically binds to the genetic switch region of Lactobacillus casei bacteriophage A2. Virology 262:220-229.
9. Martin, M. C., J. C. Alonso, J. E. Suarez, and M. A. Alvarez. 2000. Generation of food-grade recombinant lactic acid bacterium strains by site-specific recombination. Appl. Environ. Microbiol. 66:2599-2604.
10. Moscoso, M., and J. E. Suarez. 2000. Characterization of the DNA replication module of bacteriophage A2 and use of its origin of replication as a defense against infection during milk fermentation by Lactobacillus casei. Virology 273:101-111.
11. Ladero, V., P. Garcia, J. C. Alonso, and J. E. Suarez. 2002. Interaction of the Cro repressor with the lysis/lysogeny switch of the Lactobacillus casei temperate bacteriophage A2. J. Gen. Virol. 83:2891-2895.
12. Garcia, P., V. Ladero, and J. E. Suarez. 2003. Analysis of the morphogenetic cluster and genome of the temperate Lactobacillus casei bacteriophage A2. Arch. Virol. 148:1051-1070.
13. Rodriguez, I., P. Garcia, and J. E. Suarez. 2005. A second case of-1 ribosomal frameshifting affecting a major virion protein of the Lactobacillus bacteriophage A2. J. Bacteriol. 187:8201-8204.

Remarks

History
Isolé par Date
Juan E. Suárez
Área de Microbiología
Facultad de Medicina
c/Julián Clavería 6
33006 Oviedo
1982
Received from Date
Juan E. Suárez
Área de Microbiología
Facultad de Medicina
c/Julián Clavería 6
33006 Oviedo
2011-07-22
Original source
Fermented milk, Oviedo, Spain

Last update
2024-01-22
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Université Laval, QC, Canada, G1V 0A6
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